Readers ask: In 1997, Dolly The Sheep Was Cloned. Which Of The Following Processes Was Used?

Which of the following processes was used to create Dolly?

She was created using the technique of somatic cell nuclear transfer, where the cell nucleus from an adult cell is transferred into an unfertilized oocyte (developing egg cell) that has had its cell nucleus removed.

Why was the cloning of Dolly considered a major scientific breakthrough?

Dolly was important because she was the first mammal to be cloned from an adult cell. Her birth proved that specialised cells could be used to create an exact copy of the animal they came from.

What are the two types of cloning?

There are three different types of cloning:

• Gene cloning, which creates copies of genes or segments of DNA.
• Reproductive cloning, which creates copies of whole animals.
• Therapeutic cloning, which creates embryonic stem cells.

What are the 6 steps to cloning?

In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6)

Is cloning illegal?

Under the AHR Act, it is illegal to knowingly create a human clone, regardless of the purpose, including therapeutic and reproductive cloning. In some countries, laws separate these two types of medical cloning.

Is human cloning possible now?

There currently is no solid scientific evidence that anyone has cloned human embryos. In 1998, scientists in South Korea claimed to have successfully cloned a human embryo, but said the experiment was interrupted very early when the clone was just a group of four cells.

How much did it cost to clone Dolly the sheep?

At $50,000 a pet, there are unlikely to be huge numbers of cloned cats in the near future. In Britain, the idea is far from the minds of most scientists. “It’s a rather fatuous use of the technology,” said Dr Harry Griffin, director of the Roslin Institute in Edinburgh, which produced Dolly.

What are the 4 types of cloning?

Types of cloning

• Recombinant DNA Technology (DNA cloning) This is also referred to as, gene cloning, or molecular cloning.
• Reproductive Cloning. This type of cloning is used to generate an animal with the same nuclear DNA as another existing animal.
• Therapeutic cloning.

Is cloning good or bad?

A press release from the Whitehead Institute said that the study proves that no matter how normal a cloned animal may look at birth, it will likely develop health problems later in life. “Thus, cloning for the purpose of producing another human being is completely unsafe and unethical,” the release said.

What are the 4 steps of gene cloning?

The basic cloning workflow includes four steps:

• Isolation of target DNA fragments (often referred to as inserts)
• Ligation of inserts into an appropriate cloning vector, creating recombinant molecules (e.g., plasmids)
• Transformation of recombinant plasmids into bacteria or other suitable host for propagation.

How do we clone DNA?

Steps of DNA cloning

1. Cut open the plasmid and “paste” in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
2. Insert the plasmid into bacteria.
3. Grow up lots of plasmid-carrying bacteria and use them as “factories” to make the protein.

What is the difference between cloning and PCR?

Molecular cloning replicates DNA within in a living cell, while PCR replicates DNA in an in vitro solution, free of living cells. Molecular cloning involves cutting and pasting the sequences, while PCR amplifies DNA by copying an existing sequence.

What are the pros and cons of cloning?

The Pros and Cons of Cloning: Is it Worth the Risk?

• Pro: Reproductive Cloning. Reproductive cloning has a number of pros.
• Pro: Organ Replacement.
• Pro: Genetic Research.
• Pro: Obtaining Desired Traits in Organisms.
• Pro: Recovery from Traumatic Injury.
• Con: Reproductive Cloning.
• Con: Increased Malpractice.
• Con: Lack of Diversity.